Journal: Human Gene Therapy

Article Title: Benchmarking of Scale-X Bioreactor System in Lentiviral and Adenoviral Vector Production

doi: 10.1089/hum.2019.247

Figure Lengend Snippet: Cell counts of dismantled Univercells' scale-X™ hydro bioreactor. Schematic picture of scale-X bioreactor from top (a) and from the side (b) . Light gray and dark gray spots indicate the points from which samples were taken for cell density analysis. Light gray = sample of inner membrane, and dark gray = sample of outer membrane layer. For cell density analysis, nuclei were counted from top , middle and, bottom of the fixed-bed, and from the outer surface, middle , and inner surface of the rolled membrane. Cell densities (cells/cm 2 ) are shown in the table below the schematic pictures.

Article Snippet: In scale-X bioreactor runs, Univercells' (Gosselies, Belgium) 2.4 m scale-X hydro bioreactors were used.

Techniques: Membrane

Journal: Human Gene Therapy

Article Title: Benchmarking of Scale-X Bioreactor System in Lentiviral and Adenoviral Vector Production

doi: 10.1089/hum.2019.247

Figure Lengend Snippet: Cell density and glucose and lactate concentrations in scale-X hydro bioreactor and iCELLis ® Nano bioreactor runs. (a) Cell densities (cells/cm 2 ) calculated from top of the fixed-bed on days 0–4, (b) glucose and (c) lactate concentrations on days 0–7. Control Nano = Nano run, run together with scale-X bioreactor runs. Standard Nano = average of five standard Nano runs. scale-X 1–4 = scale-X bioreactor runs 1–4.

Article Snippet: In scale-X bioreactor runs, Univercells' (Gosselies, Belgium) 2.4 m scale-X hydro bioreactors were used.

Techniques: Control

Journal: Human Gene Therapy

Article Title: Benchmarking of Scale-X Bioreactor System in Lentiviral and Adenoviral Vector Production

doi: 10.1089/hum.2019.247

Figure Lengend Snippet: Scatter dot blot images of media and glucose consumption in scale-X hydro bioreactor and iCELLis Nano bioreactor runs. (a, b) Media consumption before transfections in mL (a) and in μL/cm 2 (b) . (c, d) Total media consumed during the runs until harvest in mL (c) and in μL/cm 2 (d) . (e , f) Glucose consumed until transfection in g (e) and in mg/cm 2 (f) . (g, h) Total glucose consumption until harvest in g (g) and in mg/cm 2 (h) . (i, j) Cell-specific glucose consumption in pmol/cell/day until transfection (i) and until harvest (j), taking into account that after transfection, cell density is not increased. Standard Nano = five standard iCELLis Nano runs aiming at lentiviral vector production, control Nano = run together with scale-X bioreactor runs. scale-X 1–4 = scale-X bioreactor runs 1–4, of which run 1 was not transfected, but run was stopped on day 4 to disassemble the fixed-bed. For standard Nano runs value for each runs is indicated as a separate spot; in addition, SEM and median are shown. SEM, standard error of the mean.

Article Snippet: In scale-X bioreactor runs, Univercells' (Gosselies, Belgium) 2.4 m scale-X hydro bioreactors were used.

Techniques: Dot Blot, Transfection, Plasmid Preparation, Control

Journal: Human Gene Therapy

Article Title: Benchmarking of Scale-X Bioreactor System in Lentiviral and Adenoviral Vector Production

doi: 10.1089/hum.2019.247

Figure Lengend Snippet: Lentiviral vector yields in scale-X hydro bioreactor and iCELLis Nano bioreactor runs. (a, b) Total lentiviral particles (vp) and vp/cm 2 produced, respectively, in scale-X bioreactor and iCELLis Nano bioreactor runs. (c, d) Total TU and TU/cm 2 produced, respectively, in scale-X bioreactor and iCELLis Nano bioreactor runs. Control Nano = Nano run, run together with scale-X bioreactor runs. Standard Nano = average of five standard Nano runs. scale-X 1–4 = scale-X bioreactor runs 1–4. Mean ± SEM. TU, transducing units; vp, vector particle.

Article Snippet: In scale-X bioreactor runs, Univercells' (Gosselies, Belgium) 2.4 m scale-X hydro bioreactors were used.

Techniques: Plasmid Preparation, Produced, Control